Nickel Column:Article Title: Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution
Article Snippet: .. Lane M , PageRuler Prestained Protein Ladder (labelled in kDa); lane 1, crude soluble extract; lane 2, pass-through from nickel column; lane 3, wash; lane 4, purified rGehC. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. epidermidis ATCC 12228 purchased from BCRC DNA source Template for the GehC protein cloned from the S. epidermidis genome Forward primer † GGGG CCATGG AACAAAAACAATATAAAAA Reverse primer ‡ GTGGTG CTCGAG TTTATTTGTTGATGTTAATTGTTC Cloning vector pET-28a (Novagen) Expression vector pET-28a (Novagen) Expression host E. coli BL21(DE3) Complete amino-acid sequence of the construct produced MEQKQYKNNDPIILVHGFNGFTDDINPSVLTHYWGGDKMNIRQDLEENGYEAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGVYKDWKPGQKIHLVGHSMGGQTIRQLEELLRHGNPEEVEYQKQHGGEISPLYQGGHDNMVSSITTLGTPHNGTHASDLLGNEAIVRQLAYDVGKMYGNKDSRVDFGLEHWGLKQKPNESYIQYVKRVQNSKLWKSKDSGLHDLTRDGATDLNRKTSLNPNIVYKTYTGESTHKTLAGKQKADLNMFLPFTITGNLIGKAKEKEWRENDGLVSVISSQHPFNQKYVEATDKNQKGVWQVTPTKHDWDHVDFVGQDSTDTKRTRDELQQFWHGLAEDLVQSEQLTSTNK Open in a separate window † The NcoI site is underlined. ..
Purification:Article Title: Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution
Article Snippet: .. Lane M , PageRuler Prestained Protein Ladder (labelled in kDa); lane 1, crude soluble extract; lane 2, pass-through from nickel column; lane 3, wash; lane 4, purified rGehC. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. epidermidis ATCC 12228 purchased from BCRC DNA source Template for the GehC protein cloned from the S. epidermidis genome Forward primer † GGGG CCATGG AACAAAAACAATATAAAAA Reverse primer ‡ GTGGTG CTCGAG TTTATTTGTTGATGTTAATTGTTC Cloning vector pET-28a (Novagen) Expression vector pET-28a (Novagen) Expression host E. coli BL21(DE3) Complete amino-acid sequence of the construct produced MEQKQYKNNDPIILVHGFNGFTDDINPSVLTHYWGGDKMNIRQDLEENGYEAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGVYKDWKPGQKIHLVGHSMGGQTIRQLEELLRHGNPEEVEYQKQHGGEISPLYQGGHDNMVSSITTLGTPHNGTHASDLLGNEAIVRQLAYDVGKMYGNKDSRVDFGLEHWGLKQKPNESYIQYVKRVQNSKLWKSKDSGLHDLTRDGATDLNRKTSLNPNIVYKTYTGESTHKTLAGKQKADLNMFLPFTITGNLIGKAKEKEWRENDGLVSVISSQHPFNQKYVEATDKNQKGVWQVTPTKHDWDHVDFVGQDSTDTKRTRDELQQFWHGLAEDLVQSEQLTSTNK Open in a separate window † The NcoI site is underlined. ..
Clone Assay:Article Title: Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution
Article Snippet: .. Lane M , PageRuler Prestained Protein Ladder (labelled in kDa); lane 1, crude soluble extract; lane 2, pass-through from nickel column; lane 3, wash; lane 4, purified rGehC. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. epidermidis ATCC 12228 purchased from BCRC DNA source Template for the GehC protein cloned from the S. epidermidis genome Forward primer † GGGG CCATGG AACAAAAACAATATAAAAA Reverse primer ‡ GTGGTG CTCGAG TTTATTTGTTGATGTTAATTGTTC Cloning vector pET-28a (Novagen) Expression vector pET-28a (Novagen) Expression host E. coli BL21(DE3) Complete amino-acid sequence of the construct produced MEQKQYKNNDPIILVHGFNGFTDDINPSVLTHYWGGDKMNIRQDLEENGYEAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGVYKDWKPGQKIHLVGHSMGGQTIRQLEELLRHGNPEEVEYQKQHGGEISPLYQGGHDNMVSSITTLGTPHNGTHASDLLGNEAIVRQLAYDVGKMYGNKDSRVDFGLEHWGLKQKPNESYIQYVKRVQNSKLWKSKDSGLHDLTRDGATDLNRKTSLNPNIVYKTYTGESTHKTLAGKQKADLNMFLPFTITGNLIGKAKEKEWRENDGLVSVISSQHPFNQKYVEATDKNQKGVWQVTPTKHDWDHVDFVGQDSTDTKRTRDELQQFWHGLAEDLVQSEQLTSTNK Open in a separate window † The NcoI site is underlined. ..
Cloning:Article Title: Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution
Article Snippet: .. Lane M , PageRuler Prestained Protein Ladder (labelled in kDa); lane 1, crude soluble extract; lane 2, pass-through from nickel column; lane 3, wash; lane 4, purified rGehC. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. epidermidis ATCC 12228 purchased from BCRC DNA source Template for the GehC protein cloned from the S. epidermidis genome Forward primer † GGGG CCATGG AACAAAAACAATATAAAAA Reverse primer ‡ GTGGTG CTCGAG TTTATTTGTTGATGTTAATTGTTC Cloning vector pET-28a (Novagen) Expression vector pET-28a (Novagen) Expression host E. coli BL21(DE3) Complete amino-acid sequence of the construct produced MEQKQYKNNDPIILVHGFNGFTDDINPSVLTHYWGGDKMNIRQDLEENGYEAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGVYKDWKPGQKIHLVGHSMGGQTIRQLEELLRHGNPEEVEYQKQHGGEISPLYQGGHDNMVSSITTLGTPHNGTHASDLLGNEAIVRQLAYDVGKMYGNKDSRVDFGLEHWGLKQKPNESYIQYVKRVQNSKLWKSKDSGLHDLTRDGATDLNRKTSLNPNIVYKTYTGESTHKTLAGKQKADLNMFLPFTITGNLIGKAKEKEWRENDGLVSVISSQHPFNQKYVEATDKNQKGVWQVTPTKHDWDHVDFVGQDSTDTKRTRDELQQFWHGLAEDLVQSEQLTSTNK Open in a separate window † The NcoI site is underlined. ..
Plasmid Preparation:Article Title: Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution
Article Snippet: .. Lane M , PageRuler Prestained Protein Ladder (labelled in kDa); lane 1, crude soluble extract; lane 2, pass-through from nickel column; lane 3, wash; lane 4, purified rGehC. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. epidermidis ATCC 12228 purchased from BCRC DNA source Template for the GehC protein cloned from the S. epidermidis genome Forward primer † GGGG CCATGG AACAAAAACAATATAAAAA Reverse primer ‡ GTGGTG CTCGAG TTTATTTGTTGATGTTAATTGTTC Cloning vector pET-28a (Novagen) Expression vector pET-28a (Novagen) Expression host E. coli BL21(DE3) Complete amino-acid sequence of the construct produced MEQKQYKNNDPIILVHGFNGFTDDINPSVLTHYWGGDKMNIRQDLEENGYEAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGVYKDWKPGQKIHLVGHSMGGQTIRQLEELLRHGNPEEVEYQKQHGGEISPLYQGGHDNMVSSITTLGTPHNGTHASDLLGNEAIVRQLAYDVGKMYGNKDSRVDFGLEHWGLKQKPNESYIQYVKRVQNSKLWKSKDSGLHDLTRDGATDLNRKTSLNPNIVYKTYTGESTHKTLAGKQKADLNMFLPFTITGNLIGKAKEKEWRENDGLVSVISSQHPFNQKYVEATDKNQKGVWQVTPTKHDWDHVDFVGQDSTDTKRTRDELQQFWHGLAEDLVQSEQLTSTNK Open in a separate window † The NcoI site is underlined. ..
Expressing:Article Title: Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution
Article Snippet: .. Lane M , PageRuler Prestained Protein Ladder (labelled in kDa); lane 1, crude soluble extract; lane 2, pass-through from nickel column; lane 3, wash; lane 4, purified rGehC. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. epidermidis ATCC 12228 purchased from BCRC DNA source Template for the GehC protein cloned from the S. epidermidis genome Forward primer † GGGG CCATGG AACAAAAACAATATAAAAA Reverse primer ‡ GTGGTG CTCGAG TTTATTTGTTGATGTTAATTGTTC Cloning vector pET-28a (Novagen) Expression vector pET-28a (Novagen) Expression host E. coli BL21(DE3) Complete amino-acid sequence of the construct produced MEQKQYKNNDPIILVHGFNGFTDDINPSVLTHYWGGDKMNIRQDLEENGYEAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGVYKDWKPGQKIHLVGHSMGGQTIRQLEELLRHGNPEEVEYQKQHGGEISPLYQGGHDNMVSSITTLGTPHNGTHASDLLGNEAIVRQLAYDVGKMYGNKDSRVDFGLEHWGLKQKPNESYIQYVKRVQNSKLWKSKDSGLHDLTRDGATDLNRKTSLNPNIVYKTYTGESTHKTLAGKQKADLNMFLPFTITGNLIGKAKEKEWRENDGLVSVISSQHPFNQKYVEATDKNQKGVWQVTPTKHDWDHVDFVGQDSTDTKRTRDELQQFWHGLAEDLVQSEQLTSTNK Open in a separate window † The NcoI site is underlined. ..
Sequencing:Article Title: Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution
Article Snippet: .. Lane M , PageRuler Prestained Protein Ladder (labelled in kDa); lane 1, crude soluble extract; lane 2, pass-through from nickel column; lane 3, wash; lane 4, purified rGehC. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. epidermidis ATCC 12228 purchased from BCRC DNA source Template for the GehC protein cloned from the S. epidermidis genome Forward primer † GGGG CCATGG AACAAAAACAATATAAAAA Reverse primer ‡ GTGGTG CTCGAG TTTATTTGTTGATGTTAATTGTTC Cloning vector pET-28a (Novagen) Expression vector pET-28a (Novagen) Expression host E. coli BL21(DE3) Complete amino-acid sequence of the construct produced MEQKQYKNNDPIILVHGFNGFTDDINPSVLTHYWGGDKMNIRQDLEENGYEAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGVYKDWKPGQKIHLVGHSMGGQTIRQLEELLRHGNPEEVEYQKQHGGEISPLYQGGHDNMVSSITTLGTPHNGTHASDLLGNEAIVRQLAYDVGKMYGNKDSRVDFGLEHWGLKQKPNESYIQYVKRVQNSKLWKSKDSGLHDLTRDGATDLNRKTSLNPNIVYKTYTGESTHKTLAGKQKADLNMFLPFTITGNLIGKAKEKEWRENDGLVSVISSQHPFNQKYVEATDKNQKGVWQVTPTKHDWDHVDFVGQDSTDTKRTRDELQQFWHGLAEDLVQSEQLTSTNK Open in a separate window † The NcoI site is underlined. ..
Construct:Article Title: Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution
Article Snippet: .. Lane M , PageRuler Prestained Protein Ladder (labelled in kDa); lane 1, crude soluble extract; lane 2, pass-through from nickel column; lane 3, wash; lane 4, purified rGehC. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. epidermidis ATCC 12228 purchased from BCRC DNA source Template for the GehC protein cloned from the S. epidermidis genome Forward primer † GGGG CCATGG AACAAAAACAATATAAAAA Reverse primer ‡ GTGGTG CTCGAG TTTATTTGTTGATGTTAATTGTTC Cloning vector pET-28a (Novagen) Expression vector pET-28a (Novagen) Expression host E. coli BL21(DE3) Complete amino-acid sequence of the construct produced MEQKQYKNNDPIILVHGFNGFTDDINPSVLTHYWGGDKMNIRQDLEENGYEAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGVYKDWKPGQKIHLVGHSMGGQTIRQLEELLRHGNPEEVEYQKQHGGEISPLYQGGHDNMVSSITTLGTPHNGTHASDLLGNEAIVRQLAYDVGKMYGNKDSRVDFGLEHWGLKQKPNESYIQYVKRVQNSKLWKSKDSGLHDLTRDGATDLNRKTSLNPNIVYKTYTGESTHKTLAGKQKADLNMFLPFTITGNLIGKAKEKEWRENDGLVSVISSQHPFNQKYVEATDKNQKGVWQVTPTKHDWDHVDFVGQDSTDTKRTRDELQQFWHGLAEDLVQSEQLTSTNK Open in a separate window † The NcoI site is underlined. ..
Produced:Article Title: Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution
Article Snippet: .. Lane M , PageRuler Prestained Protein Ladder (labelled in kDa); lane 1, crude soluble extract; lane 2, pass-through from nickel column; lane 3, wash; lane 4, purified rGehC. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. epidermidis ATCC 12228 purchased from BCRC DNA source Template for the GehC protein cloned from the S. epidermidis genome Forward primer † GGGG CCATGG AACAAAAACAATATAAAAA Reverse primer ‡ GTGGTG CTCGAG TTTATTTGTTGATGTTAATTGTTC Cloning vector pET-28a (Novagen) Expression vector pET-28a (Novagen) Expression host E. coli BL21(DE3) Complete amino-acid sequence of the construct produced MEQKQYKNNDPIILVHGFNGFTDDINPSVLTHYWGGDKMNIRQDLEENGYEAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGVYKDWKPGQKIHLVGHSMGGQTIRQLEELLRHGNPEEVEYQKQHGGEISPLYQGGHDNMVSSITTLGTPHNGTHASDLLGNEAIVRQLAYDVGKMYGNKDSRVDFGLEHWGLKQKPNESYIQYVKRVQNSKLWKSKDSGLHDLTRDGATDLNRKTSLNPNIVYKTYTGESTHKTLAGKQKADLNMFLPFTITGNLIGKAKEKEWRENDGLVSVISSQHPFNQKYVEATDKNQKGVWQVTPTKHDWDHVDFVGQDSTDTKRTRDELQQFWHGLAEDLVQSEQLTSTNK Open in a separate window † The NcoI site is underlined. ..
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